advantages of refractometer compared to urinometer

There are a variety of items being checked in urinalysis such as: The kidneys are the organs that produce urine. Our website services, content, and products are for informational purposes only. The buoyancy of the hydrometer needle shows the specific gravity and salinity. Urine samples less than 60 mL were measured in a smaller Assistant Urinprober hydrometer (model 248), which is graduated in intervals of 0.002 units with a scale ranging from 1.000 to 1.060. Bland-Altman plot for refractometry and hydrometry. Its also important to know that youre using a refractometer calibrated for use with seawater as many cheap refractometers are actually designed for use in brine, a simple solution of salt and water without the other elements naturally found in seawater. Finally, analysis of the data in Table Table11 and Figure Figure55 reveals that reagent-strip measurements were not consistent between trials for the testers or among testers. The apparatus shown in Figure 1 will be used. There are numerous advantages to using a refractometer: Very portable - A hand-held refractometer is easily carried. Hydrometer and reagent-strip measurements were significantly greater than refractometer measurements, a finding also shown with the Bland-Altman plots (see Figures Figures11 and and2).2). However, you have to watch out what you eat, especially strong colored foods like beets and food dyes as they can significantly change the color of your urine. Close the daylight plate and allow the water to spread across the sample plate. Oct 1, 2012 #11 Homercidal said: lettre de motivation stage avocat 3me Both analog and digital handheld refractometers work according to the critical angle principle. A refractometer is an optical device that, like a hydrometer, measures the specific gravity of your beer or wort. A refractometer can be affected by particle size, temperature, and concentration of the solution as well as light wavelength. All rights reserved. During preparticipation physical examinations, athletic trainers identified collegiate football players and monitored hydration status (n = 69 samples) during preseason practices. METHODS Twenty freshman NCAA Division II college football players provided urine samples on the initial day of preseason practice and again following one week of two-a day practices. The health care provider uses a dipstick made with a color-sensitive pad. This was expected because a key disadvantage of reagent strips is that the visual interpretation of color change on the reagent strip often is difficult and very subjective. - R. = comparison of velocity of light in air w/the velocity of light in a solution. Then, replace the cylinder filled with water back on the balance to weigh and record the mass of both cylinder and water. Refractometry brings several benefits as an analytic modality: Instruments are simple and relatively inexpensive, measurement is rapid, and quantitation is highly accurate. Swirl the urinometer slightly as it is inserted.make sure the instrument floats freely away from the sides of container. Chapter 4 Flashcards | Quizlet Advantages: B. REFRACTOMETER principle is Refractive Index or RI. Example Sentences: (1) There is no correlation between the relative supersaturation of CaOx and the SG-determination ( urinometer, test stick). Careers. Epub 2021 Mar 1. 5,7 The advantages of refractometric USG are that it can reliably and immediately be determined from a droplet of urine, using a simple and inexpensive urinary refractometer that reflects the concentration of a urine sample. WebDensity of solution and density of same amount of distilled water are compared at same temperature. Clinical refractometers, or TS Meters, are found in many hospitals and clinics. How might one measure the specific gravity of urine? It is used to check urine at home. Finally, scientists from Miles Laboratories,13 which produces N-MULTISTIX reagent strips, compared their reagent strips with refractometry in 791 nonhospitalized subjects. Bethesda, MD 20894, Web Policies Does the dipstick meet medical needs for urine specific gravity? It counts the components of urine in detail such as the type of cells present, crystal formation, cast, bacteria, mucus, and other components. Urinometer It helps assess whether the treatment the patient currently receives is effective. Free Laboratory Science Flashcards about Urinalysis Chapter 3 Method and Trial Main Effects (Mean SD). Urinalysis consists of three things: physical, chemical, and microscopic. Discuss the correction needed to compensate for high molecular substances as glucose or protein in determining the no need to correct for temp The amplifier is used to amplify the signals and that amplified signals are passed through a feedback network and generates the output. In conclusion, our results suggest that the refractometer is a reliable measure of urine specific gravity. ii. In this experiment is determine if the water and the unknown liquid has the different of the density. Epub 2021 Mar 19. 6 Can you measure final gravity with a refractometer? 2) Hold the urinometer float by the top and slowly insert it into the cylinder. Disclaimer. The urinometer, a type of hydrometer, was used for measuring the specific gravity of urine. Urinalysis can be done at home using a urine strip test readily available in the leading pharmacy or in a laboratory setting. Make sure you submit a fresh urine sample. 5 How does a refractometer work for specific gravity? If you are taking supplements, vitamins, and any other drugs, you need to inform your doctor right away as they could affect the result of the exam. However, interclass correlations may not be the appropriate statistical technique because the same variable is being correlated. It also has a quick turnaround time. The validity of a method may be determined by comparing it with another method known to be valid.23, To our knowledge, this is the first study to examine both the reliability and validity of methods commonly used to measure urine specific gravity. Each square represents a particular component such as the concentration of urine, pH level, protein in urine such as albumin, glucose/sugar, ketones (a byproduct of fat breakdown), hemoglobin, leukocyte esterase/white blood cells, bilirubin, nitrite, and urobilinogen. For men, the tip of the penis should be wiped by a sterile pad. 'Specific gravity' is a function of the number, density and weight of the solute particles present in the urine, and is used as a measure of the concentrating power of the kidney. On the negative side, refractometry brings value only when the constituents of a solution are well-known. A urine specific gravity measured by refractometer is 1.029, and the temperature of the urine is 14 degrees C. The specific gravity should be reported as: 1.027. Epub 2015 Jun 8. Urine specific gravity measurements: comparison of a new reagent strip method with existing methodologies, as applied to the water concentration/dilution tests. It is more of a qualitative test than a quantitative one. Tai YH, Tsai PC, Ho YL, Delaunay JJ, Wei PK. It might not always yield to accurate result as urine test using a test strip is time-sensitive. Refraction of light offers advantages for the determination of solute concentrations because the measurement requires no chemical alteration of the specimen. Easy to operate - Simply read the instructions that accompany the refractometer. 2001 Apr;16(4):374-82. doi: 10.1007/s004670000551. In 1997, three collegiate wrestlers died while attempting to reduce weight by dehydration.13 To prevent a recurrence of this tragedy, the National Collegiate Athletic Association (NCAA) introduced new rules in 1998 that discourage dangerous weight-cutting practices.13 These new rules include a weight-certification process that requires the determination of hydration status. Instant results - Readings are done "on the spot;" no need to wait for lab results. The purpose of this experiment is to learn how to measure mass and volume and to determine the density of water, alcohol, and a solid. Urine specific gravity measurement: reagent strip versus refractometer. The most efficient way of determining concentration is to prepare a set of standard solutions of known. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). 1 What are the advantages of using a refractometer instead of other specific gravity measurement equipment used in clinical microscopy? The absorbance obtained from the results of each test sample, then put into the equation of calibration curve in accordance with the respective raw substances, after which it would have obtained the value of molar absorptivitas. Intraclass coefficients between refractometry and hydrometry were moderate (R = .869) and low between refractometry and reagent strips (R = .573). While analog handheld refractometers require an external light source for measurement, digital refractometers use light from an internal LED light source. Urinometer. density of a substance compared to the density of distilled water at similar temperature. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. (7, 8, and 9). government site. Joined Sep 10, 2011 Messages 1,241 Reaction score 66 Location Austin. Only the refractometer should be used to determine urine specific gravity in collegiate wrestlers during the weight-certification process. COMPARISON OF URINARY OSMOMETER AND REFRACTOMETER Urine samples were divided into 2 subsamples (trials A and B) for analysis. A Laboratory Textbook of Anatomy and Physiology. What are the advantages of refractometry? A significant interaction occurred between method and trial (F2,160 = 4.079, P = .0187) (Table (Table2).2). Posted on May 31, 2022 by May 31, 2022 by Urinometer. The cookie is used to store the user consent for the cookies in the category "Analytics". The original and final readings are both needed in the same calulator. Coolant refractometers often have a readout that can also be used with car battery fluid to indicate battery condition. What does the refractometer used in urinalysis measure? Because of increased density of urine compared to that of water, the urinometer will float higher in urine than in water. You have to compare the color in the test strip to the reference for color changes on the plastic bottle container. In a routine urinalysis, the urine is checked for its appearance, concentration, and composition. WebA urine specific gravity measured by refractometer is 1.029, and the temperature of the urine is 14 degrees C. The specific gravity should be reported as: 1.027: Refractive index This cookie is set by GDPR Cookie Consent plugin. The .gov means its official. Several investigators1315 have suggested that reagent strips are an acceptable alternative to refractometry. 2. although others have reported both higher and lower refractometric results compared with biuret results. Acute dehydration is a significant health risk and can lead to heat stroke and death. official website and that any information you provide is encrypted Disadvantages of Lab-Based Urine Testing. measuring urine osmolality has many advantages (Table 1). advantages of refractometer compared to urinometer Compare dipstick testing for urine specific gravity with urinometer and refractometer testing 34. refractometer What are advantages of using a refractometer? Most refractometers are calibrated by using a sample of distilled water. These include red blood cells, white blood cells, and epithelial cells.

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